Orientation of classrooms is an important challenge in architectural design of a school. However, there is a lack of appropriate knowledge about influence of orientation on students’ perception of daylighting in classrooms. This paper presents a questionnaire survey that was conducted to compare students’ perception and satisfaction with daylighting in classrooms of a high school in Tabriz, Iran. Statistical analysis of responses was done to identify daylight factor and their relationships with satisfaction with daylighting in northern and southern classrooms. In southern classrooms, satisfaction with daylighting had significantly negative and positive correlation with perception of reflected and direct glare, respectively. In northern classrooms, perception of unified daylight and control of sunlight overheating by operable windows had significantly strong and positive correlation with satisfaction with daylighting, respectively. The students’ seating location had significant influence on satisfaction with daylighting and perception of direct glare in southern classrooms. In northern classrooms, students’ seating location caused different perception of reflected glare. The results showed that orientation did not lead to significant difference between satisfactions with daylighting in northern and southern classrooms. Although, in southern classrooms, the mean votes of satisfaction with daylighting was higher than northern classrooms.