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By application of design spectra in seismic analyses, determination of design spectra for different site conditions, magnitudes, safety levels and damping ratios will improve the accuracy of seismic analysis results. The result of this research provides different design acceleration spectra based on Iran earthquakes database for different conditions. For this purpose first a set of 146 records was selected according to causative earthquake specifications, device error modification and site conditions. Then the design acceleration spectra are determined for 4 different site conditions presented in Iranian code of practice for seismic resistant design of buildings (Standard No. 2800), different magnitudes (MsO5.5 & Ms>5.5), different damping ratios (0, 2, 5, 10, 20 percent) and also various safety levels (50% & 84%). Also this research compares the determined design spectra with those in Standard No. 2800.