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In order to investigate the flexural behaviors of RC beams after freeze-thaw cycles, compressive strength test of concrete cubes after 0, 50, 100, 125 freeze-thaw cycles were made, and static flexural experiment of 48 RC beams after 0, 50, 100, 125 freeze-thaw cycles were made. The relationships of relative compressive strength, mass loss rate, relative dynamic elastic modulus and numbers of freeze-thaw cycles were analyzed. The influences of different numbers of freeze-thaw cycles on the flexural behaviors of RC beams with different concrete grades were studied. The results show that concrete cubes’ mass, relative dynamic elastic modulus and compressive strength decrease with the increasing of freeze-thaw cycles, and high-strength grade concrete could slow down the damage caused by freeze-thaw cycles. Experimental values of test beams stiffness under short-term load were smaller than theory value. Some under-reinforced RC beams occurs over-reinforced failure mode after freeze-thaw cycles. Boundary reinforcement ratio of RC beams after certain numbers of freeze-thaw cycles was derived and its correctness was verified by experiment. Current code for design of concrete structures about crack load and ultimate load are still suitable for RC beams after freeze-thaw cycles.