In this study, the recently developed method, Tug of War Optimization (TWO), is employed for simultaneous analysis, design and optimization of Water Distribution Systems (WDSs). In this method, analysis procedure is carried out using Tug of War Optimization algorithm. Design and cost optimization of WDSs are performed simultaneous with analysis process using an objective function in order to satisfying the analysis criteria, design constraints and cost optimization. A number of practical examples of WDSs are selected to demonstrate the efficiency of the presented algorithm. The findings of this study clearly signify the efficiency of the TWO algorithm in reducing the water distribution networks construction cost.